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Rev. chil. nutr ; 35(3): 225-234, sept. 2008. ilus, tab
Article in Spanish | LILACS | ID: lil-518956

ABSTRACT

An electrophoretic and microbiological study more probable number (NMP), total (CT) and fecal coliforms (CF); aerobic-mesophils bacteria (BAM), molds and yeasts in worm's flour of Eisenia fetida was carried out. In the electrophoretic profiles using gels of standard concentration, different protein fractions with molecular weights between 39.6 and 43.5 kDa were separated. When gels with growing gradient of concentration were used, proteins with higher molecular weights (124.4 and 106.8 kDa) were separated with more detail. The protein characterization using different electrophoretic systems used in this study allows studying different protein fractions that could have a nutritional importance. The counts of BAM, CT, CF, molds and yeasts in this flour were low, indicating an innocuous product. With the use of this non-conventional resource, an interesting field of investigation begins involving food enrichment, representing an alternative at the industrial level.


Se realizó un estudio electroforético y microbio-lógico [número más probable (NMP), coliformes totales (CT) y fecales (CF); bacterias aerobias-mesófilas (BAM), mohos y levaduras] en harina de lombriz Eisenia fétida. En los perfiles electroforéticos con geles de concentración estándar se separaron diferentes fracciones proteicas con pesos moleculares comprendidos entre 39,6 y 43,5 kDa, valores similares a los reportados por otros investigadores. Cuando se usaron geles con gradiente creciente de concentración, se lograron separar en forma mas detallada proteínas con pesos moleculares más altos (124,4 y 106,8 kDa). En este estudio, la caracterización de proteínas utilizando distintos sistemas electroforéticos permitió analizar las diferentes fracciones proteicas que pudiesen tener una importancia nutricional. Los recuentos de BAM, CT, CF, mohos y levaduras en esta harina fueron bajos, lográndose un producto inocuo. Es importante destacar, que con la utilización de este recurso no convencional, se inicia un interesante campo de investigación en lo que se refiere al enriquecimiento de alimentos, representando una alternativa a nivel industrial.


Subject(s)
Bacteria, Aerobic/isolation & purification , Coliforms/analysis , Flour/microbiology , Fungi/isolation & purification , Oligochaeta , Proteins/analysis , Electrophoresis, Polyacrylamide Gel , Microbiological Techniques , Molecular Weight
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